Antibody Catalog

QYAOBIO provides antibody catalog for customers in worldwide

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QYAOBIO provides 10,000+ antibodies with various specification in multiple applications. As the original manufacturer in China, our professional technical team can design, produce and validate every antibody in-house. All these antibodies are used widely in ELISA, WB, IHC/ICC, IF, IP/Co-IP, ChIP and FC.

The optimization of antibody production is focusing on yield enhancement, antibody expression improvement, downstream process streamline. Antibody production has the crucial role in advancements of diagnostics, therapeutics, and scientific research. QYAOBIO, as the custom antibody supplier, provides comprehensive antibody development and production services, from antigen design to custom antibody generation.

CORE PRODUCTS

Catalog Antibodies

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Custom Antibody Production FAQ

Yes, we can provide them all. For antigen design, our peptide chemists can provide you with antigenicity analysis services and recommend the most immunogenic sequences with high probability of exposure. In terms of antigen production, we have extensive production experience, whether it is a gene, peptide or protein, etc., your antigen can be produced in house through synthetic or recombinant expression systems.

If you are immunizing with your own submitted antigens, the requirements vary based on the kind of protocol. For protein antigens, we recommend sending a minimum of 1-2 mg of antigen for rabbit projects and 5 mg for goat projects, with a purity >85% and the concentration needs to be at least 0.5 mg/ml. In the case of a peptide antigen, we expect 20 mg peptide (>85% purity).

Yes, our data sheets list species and applications for our validated antibodies.

There are many factors that influence whether an antibody attaches to another species. Therefore even if the sequence alignment is excellent, we cannot ensure the antibody will work in an untested species.

Antibodies are only stable at room temperature for a few days, so we recommend that you store them at -20°C. Additionally, to avoid repeated freezing and thawing, you should also aliquot the antibody according to the experimental usage, with each aliquot should not be less than 10 μL. Because of evaporation of the liquid and adsorption to the container surface, too few aliquots affect antibody concentration.

Yes. The relevant proteins excised after staining should be mixed with a small amount of buffer to prevent the gel from drying out. Since protein concentration cannot be determined once the sample is received, you need to quantify the total protein amount as accurately as possible. Sometimes denatured proteins do not adhere well to ELISA microtiter plates, thus you may also wish to provide about 200 µg of protein in a non-gel state.

We will combine the peptide with KLH as a carrier protein. The KLH-peptides can be used with any peptide from any location within the protein.

The time it takes to obtain antibodies varies with different antibodies. Approximate time from start to finish for peptide antibodies is 16-24 weeks. Protein antibodies vary depending on expression system and production difficulty. The development of hybridoma takes 4 to 6 months.

Because of the serum antibody concentration and the anti-immunogen antibodies’ affinity, the yields from affinity purification can differ significantly. For every 100 mL of sera, 2–20 mg of purified anti-immunogen antibodies can be expected.

Crude ELISA titers are expressed as the inverse of serum dilution. A powerful, specific immune response is typically indicated by titers ranging from 1:50,000 to 1:100,000. For example, as shown by a titer of 1:50 000, when the antibody is attached to the solid phase at a dilution of 1:50,000, it can effectively detect the antigen.

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