Loading Control Antibodies
QYAOBIO provides loading control antibodies for customers in worldwide
Loading control antibodies can determine equal loading quality of the samples, this is critical in the interpretation of Western Blot result. Specific proteins as loading controls are selected typically for their ubiquitous express and abundance. The right loading control antibody is determined by the molecular weight of targets, sub-cellular fraction of samples, and evaluated cell and tissue types.
Control antibodies are the vital part of assay design, this ensures the detected signals are attributed to specific antigen binding, rather than non-specific background staining. Control antibodies are applied commonly to determine background staining levels. Moreover, these antibodies can be utilized as the blocking angents before addition of primary antibodies. The common control antibodies including: loading control antibodies, isotype control antibodies.
Notice: All antibodies&proteins are only for research purposes, Not for clinical use.
Features of Loading Control Antibodies
Loading control proteins are expressed constitutively and stably at high level in most tissue and cells. Housekeeping proteins represent some loading control proteins like: GAPDH, tubulin , actin.
Loading control are selected frequently from proteins expressed by the housekeeper genes. These controls can normalize the levels of protein of interest by confirming the same protein loading across the gel. The expression levels of the loading control are constant between different sample lanes. In addition, it is critical that the selected protein as the loading control should have different molecular weight with the protein of interest. In order to avoid overlapped staining and interference to the testing result.
The main functions of loading control antibodies in Western blotting:
- Estimate the relative amounts of total protein loaded in each lane. This information is required to determine different level of the protein of interest in different samples. Duo to landing and transfer errors, or alternatives in protein expression.
- Troubleshoot in the Western blotting procedures.
QYAOBIO provides antibodies against the most common loading control proteins, the loading control antibodies are helpful for assessing western blotting efficiency, and comparing the amounts of loading protein in each well across a gel.
Mouse Loading Control Antibodies
- Name: Anti-Actin Alpha 1, Cardiac Muscle (ACTC1) Monoclonal Antibody
- Catalog Number: QY-AB40053481
- Alternative Names: ACTC, CMD1R, CMD1-R, ACT-a1
- Host: Mouse
- Source: Human
- Concentration: 1mg/ml
- Purification Method: Protein A+G
- Application: Loading Control of WB
- Name: Anti-Actin Alpha 2, Smooth Muscle (ACTa2) Monoclonal Antibody
- Catalog Number: QY-AB40053480
- Alternative Names: ACT-A2, ACTSA, ACTVS, Asma, GIG46, Cell growth-inhibiting gene 46 protein
- Host: Mouse
- Source: Human
- Concentration: 1mg/ml
- Purification Method: Protein A+G
- Application: Loading Control of WB
- Name: Anti-ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) Monoclonal Antibody
- Catalog Number: QY-AB40053489
- Alternative Names: Sodium pump subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Sodium/potassium-transporting ATPase subunit alpha-1
- Host: Mouse
- Source: Human
- Concentration: 1mg/ml
- Purification Method: Protein A+G
- Application: Loading Control of WB
- Name: Anti-Beta Actin (ACTB) Monoclonal Antibody
- Catalog Number: QY-AB40053476
- Alternative Names: Actin Beta, ACT-B, PS1TP5BP1, Actin, cytoplasmic 1, Actin, cytoplasmic 1, N-terminally processed
- Host: Mouse
- Source: Human
- Concentration: 1mg/ml
- Purification Method: Protein A+G
- Application: Loading Control of WB
- Name: Anti-Beta Actin (ACTB) Monoclonal Antibody
- Catalog Number: QY-AB40054280
- Alternative Names: Actin Beta, ACT-B, PS1TP5BP1, Actin, cytoplasmic 1, Actin, cytoplasmic 1, N-terminally processed
- Host: Mouse
- Source: Human, Mouse, Rat, Guinea pig, Canine, Porcine , Bovine, Sheep, Horse, Chicken
- Concentration: 1mg/ml
- Purification Method: Protein A+G
- Application: Loading Control of WB
- Name: Anti-Beta Catenin (β-catenin) Monoclonal Antibody
- Catalog Number: QY-AB40053492
- Alternative Names: CTNNb1, bCat, b-Cat, CTNN-B1, Catenin Beta 1, Cadherin-Associated Protein
- Host: Mouse
- Source: Human
- Concentration: 1mg/ml
- Purification Method: Protein A+G
- Application: Loading Control of WB
- Name: Anti-Beta Catenin (β-catenin) Monoclonal Antibody
- Catalog Number: QY-AB40054283
- Alternative Names: CTNNb1, bCat, b-Cat, CTNN-B1, Catenin Beta 1, Cadherin-Associated Protein
- Host: Mouse
- Source: Human,Rat
- Concentration: 1mg/ml
- Purification Method: Protein A+G
- Application: Loading Control of WB
- Name: Anti-Calnexin (CNX) Monoclonal Antibody
- Catalog Number: QY-AB40053490
- Alternative Names: CANX, CNX, IP90, P90, Major Histocompatibility Complex Class I Antigen-Binding Protein p88
- Host: Mouse
- Source: Human
- Concentration: 1mg/ml
- Purification Method: Protein A+G
- Application: Loading Control of WB
- Name: Anti-Cyclophilin A (CYPA) Monoclonal Antibody
- Catalog Number: QY-AB40054840
- Alternative Names: PPIA, CyP-A, CYPH, Peptidylprolyl Isomerase A, Peptidyl-Prolyl Isomerase A, Peptidyl-prolyl cis-trans isomerase A, Cyclosporin A-binding protein
- Host: Mouse
- Source: Human, Mouse
- Concentration: 1mg/ml
- Purification Method: Protein A+G
- Application: Loading Control of WB
Rabbit Loading Control Antibodies
- Name: Anti-ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) Polyclonal Antibody
- Catalog Number: QY-AB40053571
- Alternative Names: Sodium pump subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Sodium/potassium-transporting ATPase subunit alpha-1
- Host: Rabbit
- Source: Human
- Concentration: 500µg/ml
- Purification Method: Antigen-specific + Protein A affinity chromatography
- Application: Loading Control of WB
- Name: Anti-Beta Actin (ACTB) Polyclonal Antibody
- Catalog Number: QY-AB40053561
- Alternative Names: Actin Beta, ACT-B, PS1TP5BP1, Actin, cytoplasmic 1, Actin, cytoplasmic 1, N-terminally processed
- Host: Rabbit
- Source: Human
- Concentration: 500µg/ml
- Purification Method: Antigen-specific + Protein A affinity chromatography
- Application: Loading Control of WB
- Name: Anti-Beta Actin (ACTB) Polyclonal Antibody
- Catalog Number: QY-AB40054281
- Alternative Names: Actin Beta, ACT-B, PS1TP5BP1, Actin, cytoplasmic 1, Actin, cytoplasmic 1, N-terminally processed
- Host: Rabbit
- Source: Human, Mouse, Rat, Rabbit, Rhesus monkey, Canine, Porcine
- Concentration: 0.1-1mg/ml
- Purification Method: Antigen-specific + Protein A affinity chromatography
- Application: Loading Control of WB
- Name: Anti-Beta Catenin (β-catenin) Polyclonal Antibody
- Catalog Number: QY-AB40053559
- Alternative Names: CTNNb1, bCat, b-Cat, CTNN-B1, Catenin Beta 1, Cadherin-Associated Protein
- Host: Rabbit
- Source: Human
- Concentration: 500µg/ml
- Purification Method: Antigen-specific + Protein A affinity chromatography
- Application: Loading Control of WB
- Name: Anti-Beta Catenin (β-catenin) Polyclonal Antibody
- Catalog Number: QY-AB40054282
- Alternative Names: CTNNb1, bCat, b-Cat, CTNN-B1, Catenin Beta 1, Cadherin-Associated Protein
- Host: Rabbit
- Source: Human
- Concentration: 500µg/ml
- Purification Method: Antigen-specific + Protein A affinity chromatography
- Application: Loading Control of WB
- Name: Anti-Calnexin (CNX) Polyclonal Antibody
- Catalog Number: QY-AB40053555
- Alternative Names: CANX, CNX, IP90, P90, Major Histocompatibility Complex Class I Antigen-Binding Protein p88
- Host: Rabbit
- Source: Human
- Concentration: 500µg/ml
- Purification Method: Antigen-specific + Protein A affinity chromatography
- Application: Loading Control of WB
- Name: Anti-Extracellular Signal Regulated Kinase 1 (ERK1) Polyclonal Antibody
- Catalog Number: QY-AB40053564
- Alternative Names: HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, MAPK3, ERT2, Mitogen-Activated Protein Kinase 3, Insulin-stimulated MAP2 kinase, Microtubule-associated protein 2 kinase
- Host: Rabbit
- Source: Human
- Concentration: 500µg/ml
- Purification Method: Antigen-specific + Protein A affinity chromatography
- Application: Loading Control of WB
- Name: Anti-Extracellular Signal Regulated Kinase 1 (ERK1) Polyclonal Antibody
- Catalog Number: QY-AB40054286
- Alternative Names: HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, MAPK3, ERT2, Mitogen-Activated Protein Kinase 3, Insulin-stimulated MAP2 kinase, Microtubule-associated protein 2 kinase
- Host: Rabbit
- Source: Human
- Concentration: 500µg/ml
- Purification Method: Antigen-specific + Protein A affinity chromatography
- Application: Loading Control of WB
- Name: Anti-Extracellular Signal Regulated Kinase 2 (ERK2) Polyclonal Antibody
- Catalog Number: QY-AB40053558
- Alternative Names: MAPK1, P38, P40, ERK2, ERT1, MAPK2, P42MAPK, P42-MAPK, PRKM1, PRKM2, P41, P41mapk, P41-mapk, Mitogen-Activated Protein Kinase 1, Mitogen-activated protein kinase 2
- Host: Rabbit
- Source: Human
- Concentration: 500µg/ml
- Purification Method: Antigen-specific + Protein A affinity chromatography
- Application: Loading Control of WB
Call Us
+86(021)-50795728
+86(027)-60707970