Bacterial Protein Expression
QYAOBIO provides different bacterial protein expression for customers in worldwide
Bacterial protein expression systems have popular applications , due to the easy to culture, fast growth, high yield production, and easy scale-up. In addition, bacterial expression systems also feature low production cost and high scalability. E.coli is the most widely applied bacterial host, it is the the first choice and the established tool if applicable. E.coli expression system can produce various recombinant proteins like cytokines, enzymes, immunogens, and antibody fragments.
Bacillus subtilis is another bacterium host for protein expression, it is an FDA-designated Generally Recognized As Safe (GRAS) organism. Sine it is a Gram-positive bacterium, this system is free of endotoxin concern, it also has less condon bias than E.coli. Furthermore, this system secretes proteins into the culture media directly, this simplifies subsequent protein steps.
Bacterial Expression Service
All these features make bacterial protein system an attractive system for recombinant protein production at both laboratory and industrial levels. However, multi-domain eukaryotic proteins, expressed in bacteria cells, are often non-functional. Due to the cells are not equipped with post-translational modifications or molecular folding. In addition, many proteins become insoluble as inclusion bodies, which are difficult to recover without harsh denaturants and subsequent cumbersome protein-refolding procedures.
QYAOBIO provides ultimate solution for protein production in bacterial cell lines. Our custom service can satisfy specialized requirements, like codon optimization, tag selection, purity, endotoxin removal, protein refolding. We provide comprehensive service in bacterial expression systems, including:
- Clone genes of interest into the bacterial expression vector with His-tag or selected tag
- Amplify and isolate the gene of interest from vectors, and sub-clone into the bacterial expression vector
- Verification of the authenticity of sub-cloneed gene by restriction of enzyme digest and sequencing
- Transform recombinant constructions into high efficiency strain
- Mini-induction to over-express the target proteins
- Test expression of the recombinant proteins
Advantage of Bacterial Expression System
As the popular host choice for expression, bacterial expression systems have significant advantages as following:
- Simple physiology process
- Short generation time, rapid grow
- Tight control of gene expression
- Highest yield comparing to other systems
- Broad expression range levels in different inducer concentrations
- Active at low temperature
- Low sensitivity to media formulation
- Availability of vectors with protein fusion tags for purification
- Cost effective and scalable bio-process engineering
- Higher successful rate in obtain of soluble proteins
Disadvantage of Bacterial Expression System
Inactivity
The folding structures are often not proper in bacterial expression systems, this results in biological inactivity.
Toxicity
The synthesized proteins are usually toxic to bacteria, this prevents the cell cultures from reaching to high density. The inducible promoter is a solution to this problem.
Modification
Bacteria systems are lack of enzymes responsible for post-translational modifications with effect on protein functions. Such as: there is no apparatus in the bacterium for attachment of the necessary sugar residues. In this case, mammalian cells system is the best choice.
Common Process in E.coli Expression System
- Apply competent E.coli cell to take up DNA sequence
- Integrate the DNA into bacterial genome, circularize DNA sequence as a plasmid
- Apply the selection marker to select transformed E. coli
- Expand selected E. coli to high scale in appropriate culture media
- Isolate and purify the intracellular or secreted proteins
Our Competitive Advantage
QYAOBIO provides the cost-effective bacterial expression system platform for high quality protein production. Our expression levels in bacterial improve significantly, by application of self-developed codon optimization technology and mature expression system. You only need to provide the sequence of target proteins, then we can deliver the specified quantity and purity of target proteins.
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